資料載入中.....
|
請使用永久網址來引用或連結此文件:
http://163.15.40.127/ir/handle/987654321/393
|
| 題名: | Acetone, ethanol and methanol extracts of Phyllanthus urinaria inhibit HSV-2 infection in vitro |
| 作者: | Yang, Chien-Min;曾華友;Cheng, Hua-Yew;Lin, Ta-Chen;Chiang, Lien-Chai;Lin, Chun-Ching;(東方技術學院化妝品應用與管理系) |
| 貢獻者: | 東方技術學院化妝品應用與管理系 |
| 關鍵詞: | Anti-HSV activity;P. urinaria;Plant;Early stage of infection;Virus infectivity |
| 日期: | 2005-07 |
| 上傳時間: | 2009-11-30 15:36:17 (UTC+8) |
| 摘要: | Phyllanthus urinaria Linnea (Euphorbiaceae) is one of the traditional medicinal plants that are widely applied by oriental people, especially by Chinese and Indian, to ameliorate various kinds of ailments. Many biological activities, including anti-hepatitis B virus, anti-Epstein–Barr virus and anti-retroviral reverse transcriptase, of P. urinaria have been reported, but not against herpes simplex virus (HSV). In this study, the anti-HSV-1 and HSV-2 activities of different solvents extracted from P. urinaria were investigated in vitro by plaque reduction assay. Results showed that acetone, ethanol and methanol extracts of P. urinaria inhibited HSV-2 but not HSV-1 infection. The 50% inhibitory concentration against HSV-2 infection (IC50) of acetone, ethanol and methanol extracts was 4.3 ± 0.5, 5.0 ± 0.4 and 4.0 ± 0.9 mcg/ml, respectively. All three extracts showed no cytotoxic effect against Vero cells at concentrations of 10.0 mcg/ml or below. The time-of-addition study demonstrated that these three extracts were only effective when added during the HSV-2 infection which, therefore, suggested that they disturb the initial stage of HSV-2 infection. Furthermore, they can diminish virus infectivity without significantly affecting incubation time and temperature. Therefore, the acetone, ethanol and methanol extracts of P. urinaria were concluded to likely inhibit HSV-2 infection through disturbing the early stage of virus infection and through diminishing the virus infectivity. |
| 關聯: | Antiviral Rcscarch , Vol.67 no.1, pp.24-30 |
| 顯示於類別: | [時尚美妝設計系] 期刊論文
|
在TFIR中所有的資料項目都受到原著作權保護.
|
