 |
English
|
正體中文
|
简体中文
|
全文筆數/總筆數 : 848/2341 (36%)
造訪人次 : 5000599
線上人數 : 69
|
|
|
資料載入中.....
|
請使用永久網址來引用或連結此文件:
http://163.15.40.127/ir/handle/987654321/241
|
| 題名: | Baicalin-Induced Apoptosis is Mediated by Bcl-2-Dependent, but not p53-Dependent, Pathway in Human Leukemia Cell Lines |
| 作者: | Shieh, Den-En;曾華友;Cheng, Hua-Yew;Yen, Ming-Hong;Chiang, Lien-Chai;Lin, Chun-Ching;(東方技術學院化妝品應用與管理系) |
| 關鍵詞: | Baicalin;Apoptosis;Bcl-2;Cytochrome c;Caspase-3;T-ALL |
| 日期: | 2006 |
| 上傳時間: | 2009-10-13 09:48:42 (UTC+8) |
| 摘要: | Acute lymphoblastic leukemia (ALL), especially T-acute lymphoblastic leukemia (T-ALL), is a common childhood malignant neoplastic disorder. Chemotherapy agents, particularly those that can induce apoptosis, are the major intervening strategy in the treatment of ALL. In this study, we investigated in T-ALL cell line, CCRF-CEM, the in vitro cytotoxic effect and the mechanism of action of baicalin, a compound extracted from Scutellaria baicalensis Georgi and S. rivularis Benth (Labiateae). Results demonstrated that baicalin displayed a remarkable cytotoxic effect in CCRF-CEM, with an IC(50) value of 10.6 microg/ml. It triggered apoptotic effect by fragmentizing cellular DNA and arrested the cell cycle at G(0)/G(1) phase. Baicalin (37.5 microg/ml) had not effected the expression of p53 and Fas protein. It was shown to decline the expression of Bcl-2 (22.0 pg/ml), which consequently caused the loss (52.7%) of transmembrane potential (Delta Psi m) in the mitochondria after 72 hours of treatment. Baicalin (37.5 microg/ml) also elevated the amount of cytosolic cytochrome c (19.2 microg/ml), which finally triggered the activation of caspase-3 (50.1 pmol/min). In conclusion, baicalin was found to induce apoptosis in T-ALL cell lines through multiple pathways. This finding encourages further investigation of baicalin in its role as a potential candidate for chemotherapeutic agents in T-ALL. |
| 關聯: | The American Journal of Chinese Medicine, Vol.34 no.2, pp.245-261 |
| 顯示於類別: | [時尚美妝設計系] 期刊論文
|
在TFIR中所有的資料項目都受到原著作權保護.
|
